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d gal foxo4 dri group  (MedChemExpress)


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    MedChemExpress d gal foxo4 dri group
    <t>FOXO4-DRI</t> <t>can</t> effectively maintain vascular function in naturally aging mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β, Il-6, Cxcl15 , and Tnf-α in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 6 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.
    D Gal Foxo4 Dri Group, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d gal foxo4 dri group/product/MedChemExpress
    Average 94 stars, based on 2 article reviews
    d gal foxo4 dri group - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway"

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2025.1729166

    FOXO4-DRI can effectively maintain vascular function in naturally aging mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β, Il-6, Cxcl15 , and Tnf-α in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 6 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.
    Figure Legend Snippet: FOXO4-DRI can effectively maintain vascular function in naturally aging mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β, Il-6, Cxcl15 , and Tnf-α in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 6 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Techniques Used: Western Blot, Quantitative RT-PCR, Staining, Imaging, Two Tailed Test

    FOXO4-DRI can effectively maintain vascular function in progeroid mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of progeroid mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β , Il-6 , Cxcl15 , and Tnf-α in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.
    Figure Legend Snippet: FOXO4-DRI can effectively maintain vascular function in progeroid mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of progeroid mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β , Il-6 , Cxcl15 , and Tnf-α in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Techniques Used: Western Blot, Quantitative RT-PCR, Staining, Imaging, Two Tailed Test

    FOXO4-DRI can alleviate endothelial cell senescence induced by OGD. (A) Western blot analysis was conducted to quantify the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in senescent HUVECs following treatment with FOXO4-DRI or PBS, with n ≥ 4 per group. (B) Representative immunofluorescence analysis (green for Ki-67, blue for DAPI) was performed to assess the number of Ki-67 positive HUVECs in different groups, with n = 5 per group and scale bar = 40 μm. (C) Fluorescent staining was carried out to analyze the content and localization changes of P21 and γ-H2AX in different groups, with n = 5 per group and scale bar = 40 μm. (D) Representative SA-β-GAL staining and quantification were performed to evaluate the number of positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (E) Tube formation assays were conducted to analyze relative tube lengths in different groups, with n = 5 per group and scale bar = 400 μm. (F) Representative DHE staining and quantification were carried out to evaluate the number of DHE-positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (G) Scratch assays were performed to assess endothelial cell migration coverage at 0, 12, 24, and 36 h in different groups, with n = 5 per group and scale bar = 400 μm. Data are represented as mean ± SEM. Statistical analysis assessed by 2-way RM ANOVA. (H) RT-qPCR analysis was performed to detect mRNA levels of IL-6 , IL-8 , IL-1β , and TNF-α in different groups, with n = 3 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.
    Figure Legend Snippet: FOXO4-DRI can alleviate endothelial cell senescence induced by OGD. (A) Western blot analysis was conducted to quantify the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in senescent HUVECs following treatment with FOXO4-DRI or PBS, with n ≥ 4 per group. (B) Representative immunofluorescence analysis (green for Ki-67, blue for DAPI) was performed to assess the number of Ki-67 positive HUVECs in different groups, with n = 5 per group and scale bar = 40 μm. (C) Fluorescent staining was carried out to analyze the content and localization changes of P21 and γ-H2AX in different groups, with n = 5 per group and scale bar = 40 μm. (D) Representative SA-β-GAL staining and quantification were performed to evaluate the number of positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (E) Tube formation assays were conducted to analyze relative tube lengths in different groups, with n = 5 per group and scale bar = 400 μm. (F) Representative DHE staining and quantification were carried out to evaluate the number of DHE-positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (G) Scratch assays were performed to assess endothelial cell migration coverage at 0, 12, 24, and 36 h in different groups, with n = 5 per group and scale bar = 400 μm. Data are represented as mean ± SEM. Statistical analysis assessed by 2-way RM ANOVA. (H) RT-qPCR analysis was performed to detect mRNA levels of IL-6 , IL-8 , IL-1β , and TNF-α in different groups, with n = 3 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Techniques Used: Western Blot, Immunofluorescence, Staining, Migration, Quantitative RT-PCR, Two Tailed Test

    FOXO4-DRI can block the interaction between P53 and FOXO4. (A) The FOXO4-P53 interaction was analyzed through co-immunoprecipitation in OGD-treated HUVECs, in different groups, with n = 3 per group. (B) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (C) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (D) Western blot analysis was performed to examine the distribution of pSer46-P53 and P53 in the cytoplasm and nucleus of HUVECs in different groups. With n = 5 per group. (E) Subcellular localization of pSer46-P53 in different groups of HUVECs was analyzed using representative immunofluorescence staining (pSer46-P53 in green, DAPI in blue), with n = 5 per group, scale bar = 40 μm. (F) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of naturally aged mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. (G) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of progeroid mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: FOXO4-DRI can block the interaction between P53 and FOXO4. (A) The FOXO4-P53 interaction was analyzed through co-immunoprecipitation in OGD-treated HUVECs, in different groups, with n = 3 per group. (B) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (C) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (D) Western blot analysis was performed to examine the distribution of pSer46-P53 and P53 in the cytoplasm and nucleus of HUVECs in different groups. With n = 5 per group. (E) Subcellular localization of pSer46-P53 in different groups of HUVECs was analyzed using representative immunofluorescence staining (pSer46-P53 in green, DAPI in blue), with n = 5 per group, scale bar = 40 μm. (F) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of naturally aged mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. (G) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of progeroid mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Blocking Assay, Immunoprecipitation, Western Blot, Immunofluorescence, Staining, Two Tailed Test

    FOXO4-DRI promotes apoptosis in senescent cells. (A) Western blot analysis quantified changes in protein levels of BAX, Bcl2, and c-Caspase3 in different groups, with n = 5 per group. (B) Western blot analysis assessed changes in protein levels of BAX, Bcl2, and c-Caspase3 in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment, with n = 5 per group. (C) Representative TUNEL staining and quantitative analysis were conducted to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments, with n = 5 per group and scale bar = 100 μm. (D) Representative TUNEL staining and quantification evaluated the number of TUNEL-positive cells across different groups with n = 5 per group and scale bar = 20 μm. (E) Flow cytometry analysis was used to quantitatively assess the number of Annexin V-positive cells in different groups, with a sample size of n = 5 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: FOXO4-DRI promotes apoptosis in senescent cells. (A) Western blot analysis quantified changes in protein levels of BAX, Bcl2, and c-Caspase3 in different groups, with n = 5 per group. (B) Western blot analysis assessed changes in protein levels of BAX, Bcl2, and c-Caspase3 in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment, with n = 5 per group. (C) Representative TUNEL staining and quantitative analysis were conducted to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments, with n = 5 per group and scale bar = 100 μm. (D) Representative TUNEL staining and quantification evaluated the number of TUNEL-positive cells across different groups with n = 5 per group and scale bar = 20 μm. (E) Flow cytometry analysis was used to quantitatively assess the number of Annexin V-positive cells in different groups, with a sample size of n = 5 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Western Blot, TUNEL Assay, Staining, Flow Cytometry, Two Tailed Test

    Schematic illustration of FOXO4-DRI in alleviating endothelial cell senescence aging extends cell survival. This study demonstrates that FOXO4-DRI mitigates senescence by inhibiting the FOXO4-P53 interaction, promoting P53 phosphorylation, and inducing apoptosis in senescent cells, thereby alleviating aging-related symptoms. Content produced with BioRender; license VN28TABHO4.
    Figure Legend Snippet: Schematic illustration of FOXO4-DRI in alleviating endothelial cell senescence aging extends cell survival. This study demonstrates that FOXO4-DRI mitigates senescence by inhibiting the FOXO4-P53 interaction, promoting P53 phosphorylation, and inducing apoptosis in senescent cells, thereby alleviating aging-related symptoms. Content produced with BioRender; license VN28TABHO4.

    Techniques Used: Phospho-proteomics, Produced



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    128824 depositor resolution determination method fsc  (Thermo Fisher)
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    Thermo Fisher 128824 depositor resolution determination method fsc
    TLC analysis of glycolipids in total membrane lipid extracts from E. faecium . Glycolipid profiles of E. faecium SM1, SM1 lafB T577C, SM1Δ lafB , and SM1 Δ lafB /pAT28:: lafB mutants were compared to WT E. faecium SM1. Purified lipid standards included Gal₂DAG <t>[1,2-diacyl-3-O-(α-D-galactosyl(1→6)-β-D-galactosyl)-sn-glycerol]</t> and Glc ₁ DAG [1,2-diacyl-3-O-(α-D-glucopyranosyl)-sn-glycerol] (Avanti Polar Lipids). Glycolipid species Glc ₁ DAG, Glc ₂ DAG, and Gal ₂ DAG were identified based on migration relative to standards. Additional bands representing unknown lipid species are also indicated. The absence of glycolipid bands in the Δ lafB mutant and their restoration in the complemented strain demonstrates the role of lafB in glycolipid biosynthesis. Data are representative of three independent experiments.
    128824 Depositor Resolution Determination Method Fsc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FOXO4-DRI can effectively maintain vascular function in naturally aging mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β, Il-6, Cxcl15 , and Tnf-α in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 6 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: FOXO4-DRI can effectively maintain vascular function in naturally aging mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β, Il-6, Cxcl15 , and Tnf-α in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions of the aortas in naturally aged mice after FOXO4-DRI and PBS treatments. n = 6 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Western Blot, Quantitative RT-PCR, Staining, Imaging, Two Tailed Test

    FOXO4-DRI can effectively maintain vascular function in progeroid mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of progeroid mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β , Il-6 , Cxcl15 , and Tnf-α in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: FOXO4-DRI can effectively maintain vascular function in progeroid mice. (A) Western blot experiments were conducted and quantitatively analyzed to determine the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in the aortas of progeroid mice after FOXO4-DRI or PBS treatment. n ≥ 4 per group. (B) RT-qPCR analysis was performed to examine the mRNA levels of Il-1β , Il-6 , Cxcl15 , and Tnf-α in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 3 per group. (C) Representative SA-β-Gal staining and quantitative analysis were performed to determine the number of positive cells in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (D) Representative HE staining and quantitative analysis were performed to evaluate the thickness in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. Scale bar = 100 μm. n = 5 per group. (E) Color Doppler imaging and analysis were performed to detect and analyze the structural and blood flow conditions in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. (F) Representative DHE staining and quantitative analysis were performed to assess the ROS levels in the aortas of progeroid mice after FOXO4-DRI and PBS treatments. n = 5 per group. Scale bar = 100 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Western Blot, Quantitative RT-PCR, Staining, Imaging, Two Tailed Test

    FOXO4-DRI can alleviate endothelial cell senescence induced by OGD. (A) Western blot analysis was conducted to quantify the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in senescent HUVECs following treatment with FOXO4-DRI or PBS, with n ≥ 4 per group. (B) Representative immunofluorescence analysis (green for Ki-67, blue for DAPI) was performed to assess the number of Ki-67 positive HUVECs in different groups, with n = 5 per group and scale bar = 40 μm. (C) Fluorescent staining was carried out to analyze the content and localization changes of P21 and γ-H2AX in different groups, with n = 5 per group and scale bar = 40 μm. (D) Representative SA-β-GAL staining and quantification were performed to evaluate the number of positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (E) Tube formation assays were conducted to analyze relative tube lengths in different groups, with n = 5 per group and scale bar = 400 μm. (F) Representative DHE staining and quantification were carried out to evaluate the number of DHE-positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (G) Scratch assays were performed to assess endothelial cell migration coverage at 0, 12, 24, and 36 h in different groups, with n = 5 per group and scale bar = 400 μm. Data are represented as mean ± SEM. Statistical analysis assessed by 2-way RM ANOVA. (H) RT-qPCR analysis was performed to detect mRNA levels of IL-6 , IL-8 , IL-1β , and TNF-α in different groups, with n = 3 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: FOXO4-DRI can alleviate endothelial cell senescence induced by OGD. (A) Western blot analysis was conducted to quantify the changes in protein levels of Ki-67, Lamin B, P21, P16, and γ-H2AX in senescent HUVECs following treatment with FOXO4-DRI or PBS, with n ≥ 4 per group. (B) Representative immunofluorescence analysis (green for Ki-67, blue for DAPI) was performed to assess the number of Ki-67 positive HUVECs in different groups, with n = 5 per group and scale bar = 40 μm. (C) Fluorescent staining was carried out to analyze the content and localization changes of P21 and γ-H2AX in different groups, with n = 5 per group and scale bar = 40 μm. (D) Representative SA-β-GAL staining and quantification were performed to evaluate the number of positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (E) Tube formation assays were conducted to analyze relative tube lengths in different groups, with n = 5 per group and scale bar = 400 μm. (F) Representative DHE staining and quantification were carried out to evaluate the number of DHE-positive cells in different groups, with n = 5 per group and scale bar = 100 μm. (G) Scratch assays were performed to assess endothelial cell migration coverage at 0, 12, 24, and 36 h in different groups, with n = 5 per group and scale bar = 400 μm. Data are represented as mean ± SEM. Statistical analysis assessed by 2-way RM ANOVA. (H) RT-qPCR analysis was performed to detect mRNA levels of IL-6 , IL-8 , IL-1β , and TNF-α in different groups, with n = 3 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Western Blot, Immunofluorescence, Staining, Migration, Quantitative RT-PCR, Two Tailed Test

    FOXO4-DRI can block the interaction between P53 and FOXO4. (A) The FOXO4-P53 interaction was analyzed through co-immunoprecipitation in OGD-treated HUVECs, in different groups, with n = 3 per group. (B) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (C) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (D) Western blot analysis was performed to examine the distribution of pSer46-P53 and P53 in the cytoplasm and nucleus of HUVECs in different groups. With n = 5 per group. (E) Subcellular localization of pSer46-P53 in different groups of HUVECs was analyzed using representative immunofluorescence staining (pSer46-P53 in green, DAPI in blue), with n = 5 per group, scale bar = 40 μm. (F) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of naturally aged mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. (G) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of progeroid mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: FOXO4-DRI can block the interaction between P53 and FOXO4. (A) The FOXO4-P53 interaction was analyzed through co-immunoprecipitation in OGD-treated HUVECs, in different groups, with n = 3 per group. (B) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (C) Western blot analysis quantified changes in protein levels of pSer46-P53 and P53 in different groups, with n = 5 per group. (D) Western blot analysis was performed to examine the distribution of pSer46-P53 and P53 in the cytoplasm and nucleus of HUVECs in different groups. With n = 5 per group. (E) Subcellular localization of pSer46-P53 in different groups of HUVECs was analyzed using representative immunofluorescence staining (pSer46-P53 in green, DAPI in blue), with n = 5 per group, scale bar = 40 μm. (F) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of naturally aged mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. (G) Immunofluorescence detection and analysis of subcellular localization of pSer46-P53 and FOXO4 in aortic sections of progeroid mice (pSer46-P53 in green, FOXO4 in red, DAPI in blue), with n = 5 per group, scale bar = 20 μm. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Blocking Assay, Immunoprecipitation, Western Blot, Immunofluorescence, Staining, Two Tailed Test

    FOXO4-DRI promotes apoptosis in senescent cells. (A) Western blot analysis quantified changes in protein levels of BAX, Bcl2, and c-Caspase3 in different groups, with n = 5 per group. (B) Western blot analysis assessed changes in protein levels of BAX, Bcl2, and c-Caspase3 in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment, with n = 5 per group. (C) Representative TUNEL staining and quantitative analysis were conducted to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments, with n = 5 per group and scale bar = 100 μm. (D) Representative TUNEL staining and quantification evaluated the number of TUNEL-positive cells across different groups with n = 5 per group and scale bar = 20 μm. (E) Flow cytometry analysis was used to quantitatively assess the number of Annexin V-positive cells in different groups, with a sample size of n = 5 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: FOXO4-DRI promotes apoptosis in senescent cells. (A) Western blot analysis quantified changes in protein levels of BAX, Bcl2, and c-Caspase3 in different groups, with n = 5 per group. (B) Western blot analysis assessed changes in protein levels of BAX, Bcl2, and c-Caspase3 in the aortas of naturally aged mice after FOXO4-DRI or PBS treatment, with n = 5 per group. (C) Representative TUNEL staining and quantitative analysis were conducted to determine the number of positive cells in the aortas of naturally aged mice after FOXO4-DRI and PBS treatments, with n = 5 per group and scale bar = 100 μm. (D) Representative TUNEL staining and quantification evaluated the number of TUNEL-positive cells across different groups with n = 5 per group and scale bar = 20 μm. (E) Flow cytometry analysis was used to quantitatively assess the number of Annexin V-positive cells in different groups, with a sample size of n = 5 per group. Data are presented as mean ± SEM and analyzed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Western Blot, TUNEL Assay, Staining, Flow Cytometry, Two Tailed Test

    Schematic illustration of FOXO4-DRI in alleviating endothelial cell senescence aging extends cell survival. This study demonstrates that FOXO4-DRI mitigates senescence by inhibiting the FOXO4-P53 interaction, promoting P53 phosphorylation, and inducing apoptosis in senescent cells, thereby alleviating aging-related symptoms. Content produced with BioRender; license VN28TABHO4.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway

    doi: 10.3389/fbioe.2025.1729166

    Figure Lengend Snippet: Schematic illustration of FOXO4-DRI in alleviating endothelial cell senescence aging extends cell survival. This study demonstrates that FOXO4-DRI mitigates senescence by inhibiting the FOXO4-P53 interaction, promoting P53 phosphorylation, and inducing apoptosis in senescent cells, thereby alleviating aging-related symptoms. Content produced with BioRender; license VN28TABHO4.

    Article Snippet: In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection.

    Techniques: Phospho-proteomics, Produced

    TLC analysis of glycolipids in total membrane lipid extracts from E. faecium . Glycolipid profiles of E. faecium SM1, SM1 lafB T577C, SM1Δ lafB , and SM1 Δ lafB /pAT28:: lafB mutants were compared to WT E. faecium SM1. Purified lipid standards included Gal₂DAG [1,2-diacyl-3-O-(α-D-galactosyl(1→6)-β-D-galactosyl)-sn-glycerol] and Glc ₁ DAG [1,2-diacyl-3-O-(α-D-glucopyranosyl)-sn-glycerol] (Avanti Polar Lipids). Glycolipid species Glc ₁ DAG, Glc ₂ DAG, and Gal ₂ DAG were identified based on migration relative to standards. Additional bands representing unknown lipid species are also indicated. The absence of glycolipid bands in the Δ lafB mutant and their restoration in the complemented strain demonstrates the role of lafB in glycolipid biosynthesis. Data are representative of three independent experiments.

    Journal: mBio

    Article Title: Loss of LafB activity reverses daptomycin resistance in E. faecium

    doi: 10.1128/mbio.00715-25

    Figure Lengend Snippet: TLC analysis of glycolipids in total membrane lipid extracts from E. faecium . Glycolipid profiles of E. faecium SM1, SM1 lafB T577C, SM1Δ lafB , and SM1 Δ lafB /pAT28:: lafB mutants were compared to WT E. faecium SM1. Purified lipid standards included Gal₂DAG [1,2-diacyl-3-O-(α-D-galactosyl(1→6)-β-D-galactosyl)-sn-glycerol] and Glc ₁ DAG [1,2-diacyl-3-O-(α-D-glucopyranosyl)-sn-glycerol] (Avanti Polar Lipids). Glycolipid species Glc ₁ DAG, Glc ₂ DAG, and Gal ₂ DAG were identified based on migration relative to standards. Additional bands representing unknown lipid species are also indicated. The absence of glycolipid bands in the Δ lafB mutant and their restoration in the complemented strain demonstrates the role of lafB in glycolipid biosynthesis. Data are representative of three independent experiments.

    Article Snippet: Approximately 5 μL of each of the two standards (Gal 2 DAG: 1,2-diacyl-3- O -(α- d -galactosyl1-6)-β- d -galactosyl- sn -glycerol Avanti Polar Lipids 840524P, Glc 1 DAG: 1,2-diacyl-3-O-(α-D-glucopyranosyl)-sn-glycerol Avanti Polar Lipids 840522P) and ~15 μL of each sample was spotted on the baseline of a Silica gel 60 TLC plate.

    Techniques: Membrane, Purification, Migration, Mutagenesis